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cypX [2019-06-25 09:11:15]
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cypX [2019-06-25 09:11:15]

cytochrome P450, cyclo-l-leucyl-l-leucyl dipeptide oxidase
locus
BSU35060
pI
5.31
mw
45.31 kDa
protein length
405 aa Sequence Blast
gene length
1218 bp Sequence Blast
function
biosynthesis of the extracellular iron chelator pulcherrimin
product
cytochrome P450, cyclo-l-leucyl-l-leucyl dipeptide oxidase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.3|Acquisition of iron / Other]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.1|Acquisition of iron / Other]

    • Gene

    Coordinates
    3,602,588 → 3,603,805

    The protein

    Catalyzed reaction/ biological activity

  • implicated in the three-step oxidative transformation of the diketopiperazine cyclo-l-leucyl-l-leucyl into pulcherriminic acid [Pubmed|20690619]

    • Protein family

  • [SW|cytochrome P450] family (according to Swiss-Prot)

    • Structure

  • [PDB|3NC3] [Pubmed|20690619]

    • Expression and Regulation

    Operons

    genes
    [gene|BCE1D8CDDF07396583FF50C4C192785545244BE2|yvmC]-[gene|D63EDBB6AA33708E8352F6208A9BA18CAEE0DC53|cypX]
    description
    [http://www.liebertonline.com/doi/pdf/10.1089/ind.2006.2.66 Reference]

    regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|0D555F2AB7DC863E6FF388888308E980514DB719|PchR]: repression, in [regulon|0D555F2AB7DC863E6FF388888308E980514DB719|PchR regulon]

    • regulation

  • expression of the operon is strongly induced at the beginning of [SW|biofilm formation] [pubmed|31113899]
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    Biological materials

    Mutant

  • MGNA-A333 (cypX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/333 NBRP B. subtilis, Japan]
  • BKE35060 (Δ[gene|D63EDBB6AA33708E8352F6208A9BA18CAEE0DC53|cypX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGGCTCATGTTTTACTCCC, downstream forward: _UP4_TAATAGAATTCCAAAGGTCT
  • BKK35060 (Δ[gene|D63EDBB6AA33708E8352F6208A9BA18CAEE0DC53|cypX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGGCTCATGTTTTACTCCC, downstream forward: _UP4_TAATAGAATTCCAAAGGTCT

    • References

  • 20690619,20817675,4204912,27542896